Catalog Number - 40008-05P-2

The 40008‑05P‑2 Cogent Bidentate C8™ HPLC Column is a short‑length, narrow‑bore analytical column designed for applications requiring alternative reversed‑phase selectivity with fast run times and LC‑MS compatibility. Manufactured using Cogent TYPE‑C™ silica hydride technology, this column delivers excellent selectivity, rapid equilibration, and extremely robust column lifetime compared with conventional silica‑based C8 phases.

The stationary phase consists of an aliphatic C8 hydrocarbon chain bonded via two direct silicon–carbon bonds (bidentate attachment) to a silica hydride surface. This chemistry produces a phase that is more hydrophobic than traditional C8 columns, yet less hydrophobic than Bidentate C18™, providing a useful middle ground when C18 retention is excessive. Unlike conventional C8 columns, this phase can also retain select hydrophilic compounds if desired.

The 2.1 mm internal diameter supports low‑flow operation, reduced solvent consumption, and enhanced detector sensitivity, making this column a popular choice for LC‑MS workflows. The short 50 mm length enables rapid separations and high sample throughput, provided the system can deliver precise low‑flow control and uses reduced‑volume detector flow cells.

Why Choose Bidentate C8™ Over Conventional C8

The Bidentate C8™ stationary phase differs fundamentally from standard C8 chemistry:

• Direct Si–C bonding enhances chemical stability and column lifetime

• Reduced silanol activity minimizes secondary interactions

• Greater hydrophobicity than traditional C8, but less than C18

• Stable at very low pH (below pH 2) without column degradation

• Compatible with 100 % aqueous mobile phases

These advantages expand the method‑development window beyond what standard silica‑based C8 columns can support.

Key Features

Cogent TYPE‑C™ Silica Hydride Technology

• Non‑traditional reversed‑phase surface chemistry

• Faster equilibration than conventional silica‑based C8 columns

• Extremely robust lifetime and reproducibility

Bidentate C8™ Bonding

• Two‑point attachment via direct Si–C bonds

• Alternative selectivity when C18 is overly retentive

• Balanced hydrophobic retention with extended method flexibility

Fast, Low‑Flow Analytical Geometry

• 2.1 mm ID × 50 mm length

• Short run times and high throughput

• Reduced solvent consumption

LC‑MS‑Friendly Format

• Ideal for ESI‑MS and LC‑MS/MS workflows

• Enhanced sensitivity at reduced flow rates

• Requires precise low‑flow pumping and small‑volume flow cells

System Requirements

For optimal performance with this narrow‑bore column:

• Use a pump capable of accurate and reproducible low‑flow delivery

• Employ detector flow cells designed for low internal volumes

Typical Applications

• LC‑MS and LC‑MS/MS

• Rapid analytical separations

• Compounds where C18 retention is excessive

• Alternative reversed‑phase selectivity

• Low‑pH or aqueous‑rich mobile‑phase methods

• High‑throughput screening workflows